Additional Information:

Name Related Product Names Gene Symbol Gene Fullname Gene id Alias Symbols Protein Name Description of Target Swissprot ID Species Reactivity Assay Info Sensitivity Kit Range Homology Standard Range Sample Type Product Format Specimen/Volumes Applications Reconstitution and Storage Reactivity Kit Size Kit Principle Kit Component Kit Duration Storage Lead Time Intended Use Additional Information ::
Human Ferritin ELISA Kit
Human Ferritin ELISA Kit
FTL
ferritin light chain
2512
FTL, Ferritin light chain, FTL1
Ferritin light chain
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in c
P02792
Human
Assay Methodology: Quantitative Sandwich ELISA
0.745 ng/ml
1.56 ng/ml - 100 ng/ml
Human
Refer To Manual
Serum, plasma and other biological fluids
In Solution
Refer To Manual
ELISA
1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-Ferritin conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-HUMAN FERRITIN ELISA MICRO PLATEAnti-Human Ferritin coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. HUMAN FERRITIN CALIBRATORLong Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Human
96 well
Genway Biotech Ferritin ELISA Kit (Human) (GWB-F4BE8D) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Ferritin light chain has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Ferritin light chain - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ferritin light chain captured in the well.
ComponentAmount
Anti-Human Ferritin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Ferritin Calibrator1
80 Minutes
Refer to kit instructions for component storage
Domestic: within 1 week deliveryInternational: 1 week
Research Use Only
Intended Use: The Ferritin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ferritin in serum or plasma samples of humans.
Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Human Ferritin antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-HUMAN FERRITIN ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human Ferritin.7. HUMAN FERRITIN CALIBRATOROne vial containing 1.0 ml of Human Ferritin Calibrator.