您知悉并同意,丁香通平台展示产品并非为平台自身产品,您发起询价或试用申请后,平台将会把您已提供或按要求提供的相关信息同步提供至所咨询的具体产品商家,由商家为您提供具体产品的价格咨询或产品试用注意事项及相关试用产品或完成相应购买,因此您知悉并授权平台将您的信息进行同步共享。您有权拒绝,但您知悉将无法参加询价或试用活动。 Additional Information Name Reg3g ELISA Kit (Mouse) (GWB-KBBDU3) Kit Range 31.2 - 2,000 pg/mL Sensitivity 11.7 pg/mL Sample Type serum, plasma and other biological fluids Kit Detection Colorimetric, OD 450 nm Gene Symbol REG3G Gene id 19695 Alias Symbols AI449515, Pancreatitis-associated protein 3, Pap3, REG-3-gamma, Regenerating islet-derived protein 3-gamma, Regenerating islet-derived protein III-gamma, RegIII (gamma), Reg III-gamma Swissprot ID O09049 Protein Name regenerating islet-derived protein 3-gamma Description of Target Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and Kit Principle Genway Biotech Reg3g ELISA Kit (Mouse) (GWB-KBBDU3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Reg3g has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Reg3g is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Reg3g captured in the well. Kit Component Component Amount Anti-SEMA4A Microplate 96 Wells (12 x 8 Well Strips) SEMA4A Lyophilized Standard 2 x 2 ng 100X Biotinylated SEMA4A Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Protein Accession Num NP_035390 Nucleotide Accession Num NM_011260 Assay Info Assay Methodology: Quantitative Sandwich ELISA Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date. Kit Duration ~ 3 Hours Kit linearity Sample 1:2 Dilution 1:4 Dilution 1:8 Dilution 1:16 Dilution Serum (n=5) 94-102% 78-101% 98-105% 95-105% EDTA Plasma (n=5) 87-104% 97-105% 93-101% 85-99% Heparin Plasma (n=5) 84-103% 94-101% 84-104% 93-101% Kit Recovery Matrix Recovery Range (%) Average (%) Serum (n=5) 78-97 81 EDTA Plasma (n=5) 80-89 80 Heparin Plasma (n=5) 78-93 85 Datasheets/Manuals Printable datasheet for GWB-KBBDU3 风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。