Additional Information:

Name Related Product Names Alias Symbols Protein Name Species Reactivity Assay Info Sensitivity Kit Range Homology Standard Range Sample Type Product Format Specimen/Volumes Applications Reconstitution and Storage Reactivity Kit Size Kit Principle Kit Component Kit Duration Storage Lead Time Intended Use Additional Information ::
Bovine IgA ELISA
IgA, Immunoglobulin A, Cow IgA, Bovine Immunoglobulin A, Cow Immunoglobulin A
IGHA1, IGHA2, IgA, Ig alpha 1 chain C region, Ig alpha 2 chain C region
IgA
Cow
Assay Methodology: Quantitative Sandwich ELISA
7.009 ng/ml
12.5 ng/ml - 400 ng/ml
Cow
Refer To Manual
Serum, plasma and other biological fluids
In Solution
Refer To Manual
ELISA
1. DILUENTThe 20X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-IgA conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-BOVINE IgA ELISA MICRO PLATEAnti-Bovine IgA coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. BOVINE IgA CALIBRATORLong Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Bovine
96 well
Genway Biotech IgA ELISA Kit (Bovine) (GWB-374Z03) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgA - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgA captured in the well.
ComponentAmount
Anti-Bovine IgA Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Bovine IgA Calibrator1
90 Minutes
Refer to kit instructions for component storage
Domestic: within 1 week deliveryInternational: 1 week
Research Use Only
Intended Use: The total Bovine IgA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in bovine biological samples.
Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 20X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Bovine IgA antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-BOVINE IgA ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Bovine IgA.7. BOVINE IgA CALIBRATOROne vial containing Bovine IgA calibrator.