展开引用远来拾霓 谢谢老师!1. high control 和 low control 的作用是不是要求所测标本OD值在 high control 和 low control 的OD值之间?但是我做出来有一个浓度最高的标准品OD值比high control 的OD值还高,是我检测有问题吗?2. 试剂说明书上还有两个指标,LLOQ: The lower limit of quantitation (LLOQ) for the XXX assay is 0.016 ng/mL, the lowest concentration on the standard curve that met internal criteria for accuracy and precision.ULOQ: The upper limit of quantitation (ULOQ) for the XXX assay is 3.181 ng/mL, the highest concentration on the standard curve that met internal criteria for accuracy and precision.Diluted samples with a concentration above this limit should be retested at a higher dilution.When test specimens yield A450 values greater than the ULOQ or less than the LLOQ, obtain a more accurate XXX concentration by re-assaying the test specimens at a different dilution so the new A450 values will be within these limits. 意思是当检测标本浓度在LLOQ和ULOQ之外时,需要重新配液稀释。请问这两个指标和high control、low control 有区别吗?谢谢!......我回答第一个吧,高低值只做质控并非限制你的检测上下限,所以你的结果可信。我比较懒,不愿意看英文,嘿嘿你自己翻译就好了。
limichuan 2014-05-01