产品编号：GWB-5581F8
市 场 价：¥9700.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
邮 箱：
info@ebiomall.com
商品介绍
Description:
Description:HumanSMURF2(GenBankAccessionNo.NM_022739),amino-acids130-end,withN-terminalFLAG-tag,MW=72kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.SpecificActivity:0.00049pmol/min/µgAssayconditions:50µlreactionmix(4mMATP,5mMMgCl2,1mMDTT,E1(0.5µg),E2(2.8µg),Biotin-Ubiquitin(0.5µg)andSmurf2(0-2µg)in50mMTrispH7.4)incubatefor30minat37°C,thenaddtostreptavidincoatedplatesandincubatefor30minat37°C.AddantibodyagainstSmurf2,incubate1h.Finally,incubate1hwiththeEuropium-labeledsecondaryantibody.Timeresolvedfluorescencemeasuredatroomtemperature(&lamBDa;exc=330nm,λem=620nm).Application:UsefulinconjunctionwithE1andE2forthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.Host:baculovirus
Tag:N-terminalFLAG-tag,
GINum:NM_022739
MW:72kDa
Format:aqueousbuffersolution
Formula:40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and80?g/mlFLAGpeptide
ScientificCategory:UbiquitinE3ligase
Species:human
AdditionalInformation:
| Name | SMURF2,ActiveHumanRecombinantProtein |
|---|---|
| RelatedProductNames | SMURF2,ActiveHumanRecombinantProtein |
| MolecularWeight | 72kDa |
| Purity | 40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and80?g/mlFLAGpeptide |
| Source | baculovirus |
| GINumber | NM_022739 |
| Format | AqueousBufferSolution |
| Reactivity | Human |
| Storage | -80°C |
| IntendedUse | ResearchUseOnly |
