Description:
flashBACGOLD™providessuperiorlevelsofexpressionforanyproteinthatisdestinedforsecretionortobeinsertedinthemembrane,orforanyproteinthatmightbeparticularlyliabletodegradation.
flashBACGOLD™isabaculovirusexpressionvectorthathasbeendesignedtoreduceproteolysis,maximiseproteinsecretionandimprovemembraneproteintargetingandisbasedonourpatentedflashBAC™system,removingthenecessityforplaque-purification.ThedeletionofbothchiAandv-cathgenesfromflashBACGOLD™hasimprovedtheefficacyofthesecretorypathwayandresultedinagreatlyenhancedyieldofrecombinantproteinsthataresecretedormembranetargeted(incomparisontorecombinantvirusesthatencodechiAandv-cath).v-cathencodesacysteineprotease.Thereforedeletionofthisgeneresultsinthesignificantreductionofdegradationofprotease-sensitivetargets.Itisalsoback-compatIBLewithallexistingbaculovirustransfervectorsbasedonhomologousrecombinationininsectcellsatthepolyhedrinlocus.
AdditionalInformation:
| Name | FlashBACGold-BaculovirusExpressionSystem(24reactions) |
|---|---|
| RelatedProductNames | FlashBACGold-BaculovirusExpressionSystem(24reactions) |
| IntendedUse | ResearchUseOnly |
