Species:Human
SampleType:Serum
ForthequantitativedeterminationofIGMAntibodiestoCytomegalovirus(CMV)inserum.DNAVirusInfections,Infection,Blood,PathologicProcesses,PregnancyComplications
ProductName:IGMAntibodiestoCytomegalovirus(CMV)
SampleType:Serum
#ofSamples:1X96Assays
IntendedUse:TheCMVIgMELISAisintendedforuseinthedetectionofIgMantibodiestoCytomegalovirus(CMV)infectioninhumanserum.
Introduction:CytomegalovirusisaherpesvirusandaleADIngBIOLOGicalfactorcausingcongenitalabnormalitiesandcomplicationsamongthosewhoreceivemassivebloodtransfusionsandimmunosuppressivetherapy.Abouthalfthepregnantwomenwhocontractaprimaryinfectionspreadthediseasetotheirfetus.Whenacquiredin-utero,theinfectionmaycausementalretardation,blindness,and/ordeafness.
SEROlogicaltestsfordetectingthepresenceofantibodytoCMVcandiagnoseactiveorrecentinfectionandprovidevaluableinformationregardingthehistoryofpreviousinfection.Thesetestsarealsousefulinscreeningbloodfortransfusionsinnewbornsandimmuno-compromisedrecipients.TheCMVIgMELISAisanaccurateserologicmethodtodetectCMVIgMantibodyforidentificationofCMVinfection.
Principleofthetest:PurifiedCMVantigeniscoatedonthesurfaceofmicrowells.Dilutedserumisaddedtothewells,andtheCMVIgMspecificantibody,ifpresent,bindstotheantigen.Allunboundmaterialsarewashedaway.HRP-conjugateisadded,whichbindstotheantibody-antigencomplex.ExcessHRP-conjugateiswashedoffandasolutionofTMBReagentisadded.Theenzymeconjugatecatalyticreactionisstoppedataspecifictime.TheintensityofthecolorgeneratedisproportionaltotheamountofIgMspecific-antibodyinthesample.Theresultsarereadbyamicrowellreadercomparedinaparallelmannerwithcalibratorandcontrols.
LimitationoftheTest:1.ReliableandreproducIBLeresultswillbeobtainedwhentheassayprocedureiscarriedoutwithacompleteunderstandingofthepackageinsertinstructionsandwithadherencetogoodlaboratorypractice.
2.Thewashprocedureiscritical.Insufficientwashingwillresultinpoorprecisionandfalselyelevatedabsorbancereadings.
3.Serumsamplesdemonstratinggrosslipemia,grosshemolysis,orturbidityshouldnotbeusedwiththistest.
Precision:AstudywasperformedtodocumenttypicalassayprecisionwiththeGenWayBiotechCMVIgMEIAproduct.Themean,SD,and%CVwerecalculatedforIntra-andInter-AssayinO.D.value(A450).
ProductName:IGMAntibodiestoCytomegalovirus(CMV)
SubcellularLocation:Isoform1:Secreted.Note=Duringdifferentiation,B-lymphocytesswitchfromexpressionofmembrane-boundIgMtosecretionofIgM.
SubcellularLocation:Isoform2:Cellmembrane;Single-passtypeImembraneprotein.
Polymorphism:All4combinationsoftheS/GandV/Gpolymorphismsatpositions191and216havebeenobservedinhumanmuchains.
Name | IGMAntibodiestoCytomegalovirus(CMV)ELISAQuantitationKit |
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RelatedProductNames | CMV3;IgMAntibodiestoCytomegalovirus(CMV)ELISAKit;N/AIgMAntibodiestoCytomegalovirus(CMV)CMV |
MolecularWeight | 49307 |
SwissProtNumber | P01871 |
IntendedUse | ResearchUseOnly |