产品编号：GWB-11207A
市 场 价：¥7580.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
邮 箱：
info@ebiomall.com
商品介绍
AdditionalInformation:
| Name | HumanFibrinogenELISAKit | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RelatedProductNames | HumanFibrinogenELISAKit | ||||||||||||||||
| GeneSymbol | FGA | ||||||||||||||||
| GeneFullname | fibrinogenalphachain | ||||||||||||||||
| Geneid | 2243 | ||||||||||||||||
| AliasSymbols | Fibrinogenalphachain,FGA | ||||||||||||||||
| ProteinName | Fibrinogenalphachain | ||||||||||||||||
| DescriptionofTarget | Cleavedbytheproteasethrombintoyieldmonomerswhich,togetherwithfibrinogenbeta(FGB)andfibrinogengamma(FGG),polymerizetoformaninsolublefibrinmatrix.Fibrinhasamajorfunctioninhemostasisasoneoftheprimarycomponentsofbloodcl | ||||||||||||||||
| SwissprotID | P02671 | ||||||||||||||||
| SpeciesReactivity | Human | ||||||||||||||||
| AssayInfo | AssayMethodology:QuantitativeSandwichELISA | ||||||||||||||||
| Sensitivity | 2.088ng/ml | ||||||||||||||||
| KitRange | 12.5ng/ml-400ng/ml | ||||||||||||||||
| Homology | Human | ||||||||||||||||
| StandardRange | ReferToManual | ||||||||||||||||
| SampleType | Serum,plasmaandotherBIOLOGicalfluids | ||||||||||||||||
| ProductFormat | InSolution | ||||||||||||||||
| Specimen/Volumes | ReferToManual | ||||||||||||||||
| Applications | ELISA | ||||||||||||||||
| ReconstitutionandStorage | 1.DILUENT The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C. 2.WASHSOLUTION The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C. 3.ENZYME-ANTIBODYCONJUGATE UndilutedhorserADIshperoxidaseanti-FIBconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours. 4.CHROMOGEN-SUBSTRATESOLUTION TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 5.STOPSOLUTION TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate. 6.ANTI-HUMANFIBRINOGENELISAMICROPLATE Anti-HumanFIBcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack. 7.HUMANFIBRINOGENCALIBRATOR ThelyophilizedHumanFibrinogencalibratorshouldbestoredat4Corfrozenuntilreconstituted.Thereconstitutedcalibratorshouldbealiquotedoutandstoredfrozen(Avoidmultiplefreeze-thawcycles).Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours. | ||||||||||||||||
| Reactivity | Human | ||||||||||||||||
| KitSize | 96well | ||||||||||||||||
| KitPrinciple | GenWayBiotechFibrinogenELISAKit(Human)(GWB-11207A)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforFibrinogenalphachainhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-Fibrinogenalphachain-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleFibrinogenalphachaincapturedinthewell. | ||||||||||||||||
| KitComponent |
| ||||||||||||||||
| KitDuration | 100Minutes | ||||||||||||||||
| Storage | Refertokitinstructionsforcomponentstorage | ||||||||||||||||
| LeadTime | Domestic:within1weekdelivery International:1week | ||||||||||||||||
| IntendedUse | ResearchUseOnly | ||||||||||||||||
| AdditionalInformation | IntendedUse:TheFibrinogentestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringFibrinogeninbiologicalsamplesofhumans. | ||||||||||||||||
| :: | Reagents: 1.DILUENTCONCENTRATE(RunningBuffer) Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer. 2.WASHSOLUTIONCONCENTRATE Onebottlecontaining50mlofa20Xconcentratedwashsolution. 3.ENZYME-ANTIBODYCONJUGATE100X Onevialcontaining150ulofaffinitypurifiedanti-HumanFibrinogenantibodyconjugatedwithhorseradishperoxidaseinastABIlizingbuffer. 4.CHROMOGEN-SUBSTRATESOLUTION Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3. 5.STOPSOLUTION Onevialcontaining12ml0.3Msulfuricacid. WARNING:Avoidcontactwithskin. 6.ANTI-HUMANFIBRINOGENELISAMICROPLATE Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-HumanFIB. 7.HUMANFIBRINOGENCALIBRATOR OnevialcontainingalyophilizedHumanFibrinogencalibrator. |
