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商品介绍
Description:
| ProductName | ToxoplasmagondiiIgGELISA. |
|---|---|
| #ofSamples | 1x96Assays. |
| IntendedUse | TheToxoplasmagondiiIgG-ELISAisintendedforthequalitativeandquantitativedeterminationofIgGclassantibodiesagainstToxoplasmagondiiinhumanserumorplasma(citrate). |
| Introduction | Toxoplasmagondiiisasmallintracellularparasite,whoselivecyclehasasexualandanasexualphase.Sexualdevelopmentisrestrictedtotheintestinalcellsof(probablyexclusively)cats;theoocystsformedareexcretedandduetotheirresistantcellwallstheymaybeinfectiousunderadvantageouscircumstancesforatleast1year.Animalsandmanareintermediatehostsforthe asexualproliferationofT.gondii:theingestedparasiteswillproliferateexplosivelywithinthehostcellslysingthemeventually.Theydisseminatethroughoutthebodyviacirculationandlymphaticsystemandthoughmayinfectanycelltype.Inmuscleandbraincellscystsareformedwhicharespheroidalandabout5-100μmindiameter.Cystsarevirtuallyimmortalintheintermediatehost. Toxoplasmagondiiisthemostcommonparasiteinhumans,butitsabundance(7-80%)ishighlydependentonthegeographicarea,thesocio-economicstatusandthenutritionalcustoms.Infectiononlyrarelycausestoxoplasmosisandusuallyclinicalsymptomsareabsent,butmayproducesevereproblemsinimmunosuppressedpersonsandfetus.Becauseonlyaprimaryinfectionduringpregnancymaybedangerousandevenfatalfortheunborn(theprobABIlityofcongenitalinfectionisabout50%),therecentonsetofaninfectionmustbeexcluded.Inpregnantwomeninover98%ofcases,theabsenceofIgMexcludesthepossibilityofrecentinfection.Innewbornstheverypresenceofanti-toxoplasmaIgMissufficienttoconfirmacongenitaltoxoplasmosis,sincematernalIgM,unlikeIgG,doesnotcrosstheplacentalbarrier.ButasignificantnumberofinfectedinfantsdonotdevelopdetectableIgMlevelsandthusarefalsenegative.Inimmunosuppressedpatientstoxoplasmosiscausesseverecomplicationsmostlybyreactivationofanearlierlatentinfection. Infectionmaybeidentifiedby
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| Principlesoftheassay | ThequantitativeimmunoenzymaticdeterminationofIgG-classantibodiesagainstToxoplasmagondiiisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique. MicrotiterstripwellsareprecoatedwithinactivatedToxoplasmagondiiantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedToxoplasmagondiispecificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct. TheintensityofthisproductisproportionaltotheamountofToxoplasmagondiispecificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader. |
| StorageandStability | Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2..8°C. |
| LimitationsoftheTest | Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata. Inimmunocompromizedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue. |
| References | A.Balsarietal.ELISAforToxoplasmaantibodydetection:acomparisionwithotherserodiagnostictests.J.Clin.Pathol.,33:640(1980)M.H.Beaman,R.E.McCabe,S-Y.Wong,J.S.Remington,Toxoplasmagondii,Inc:PrinciplesandPracticeofInfectiousDiseases,G.L.Mandell,J.E.Bennet,R.Dolin,eds.,ChurchillLivingstonePubl.,Fourthedition,p.2455-2475(1995)A.Bondioloetal.ELISAforToxoplasmaantibodies.Inc:ImmunoenzymeAssayTechniques,R.Malvanoed.,M.NijhoffPubl.(1980)M.E.Camargoet.al.ImmunoglobineGandimmunoglobulinMenzyme-linkedimmunosorbentassayanddefinedtoxoplasmosisserologicalpatterns.Inf.Immun.,21:55(1978)Y.Carlieretal.Evaluationoftheenzyme-linkedimmunosorbentassay(ELISA)andotherserologicaltestsforthediagnosisoftoxoplasmosis.Bull.WorldHealthOrganization,58(1):99(1980) |
AdditionalInformation:
| Name | ToxoplasmagondiiIgGquantitative |
|---|---|
| RelatedProductNames | ToxoplasmagondiiIgGELISA |
| MolecularWeight | 0.5 |
| Storage | Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C. |
| IntendedUse | ResearchUseOnly |
